Castello, Alfredo and Fischer, Bernd and Eichelbaum, Katrin and Horos, Rastislav and Beckmann, Benedikt M and Strein, Claudia and Davey, Norman E and Humphreys, David T and Preiss, Thomas and Steinmetz, Lars M and Krijgsveld, Jeroen and Hentze, Matthias W (2012) Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell, 149 (6). pp.1393-406. ISSN 1097-4172 (OA)
Full text not available from this repository.Abstract
RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.
(NHMRC grants 514904, 573731, and 632757; ARC DP0878224)
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 04 Jan 2016 03:28 |
| Last Modified: | 28 Feb 2016 22:08 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/73 |
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