Martinac, Boris and Rohde, Paul R and Cranfield, Charles G and Nomura, Takeshi (2013) Patch clamp electrophysiology for the study of bacterial ion channels in giant spheroplasts of E. coli. Methods in Molecular Biology, 966. pp.367-80. ISSN 1940-6029 (Book Chapter)
Full text not available from this repository.Abstract
Ion channel studies have been focused on ion channels from animal and human cells over many years. Based on the knowledge acquired, predominantly over the last 20 years, a large diversity of ion channels exists in cellular membranes of prokaryotes as well. Paradoxically, most of what is known about the structure of eukaryotic ion channels is based on the structure of bacterial channels. This is largely due to the suitability of bacterial cells for functional and structural studies of biological macromolecules in a laboratory environment. Development of the "giant spheroplast" preparation from E. coli cells was instrumental for functional studies of ion channels in the bacterial cell membrane. Here we describe detailed protocols used for the preparation of giant spheroplasts as well as protocols used for the patch-clamp recording of native or heterologously expressed ion channels in E. coli spheroplast membrane.
(No funders listed)
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 22 Dec 2015 04:17 |
| Last Modified: | 22 Mar 2016 04:18 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/69 |
Actions (login required)
 |
View Item |