Walton, Sarah-Jane and Lewis, Amy and Jeffery, Rosemary and Thompson, Hannah and Feakins, Roger and Giannoulatou, Eleni and Yau, Christopher and Lindsay, James O and Clark, Susan K and Silver, Andrew (2016) Familial adenomatous patients with desmoid tumours show increased expression of miR-34a in serum and high levels in tumours. Oncoscience, 3 (5-6). pp.173-85. ISSN 2331-4737 (OA)
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Abstract
Familial adenomatous polyposis (FAP) is rare affecting 1 in 10,000 people and a subset (10%) are at risk of myofibroblastic desmoid tumours (DTs) after colectomy to prevent cancer. DTs are a major cause of morbidity and mortality. The absence of markers to monitor progression and a lack of treatment options are significant limitations to clinical management. We investigated microRNAs (miRNA) levels in DTs and serum using expression array analysis on two independent cohorts of FAP patients (total, n=24). Each comprised equal numbers of patients who had formed DTs (cases) and those who had not (controls). All controls had absence of DTs confirmed by clinical and radiological assessment over at least three years post- colectomy. Technical qPCR validation was performed using an expanded cohort (29 FAP patients; 16 cases and 13 controls). The most significant elevated serum miRNA marker of DTs was miR-34a-5p and in-situ hybridisation (ISH) showed most DTs analysed (5/6) expressed miRNA-34a-5p. Exome sequencing of tumour and matched germline DNA did not detect mutations within the miR-34a-5p transcript sites or 3'-UTR of target genes that would alter functional miRNA activity. In conclusion, miR-34a-5p is a potential circulatory marker and therapy target. A large prospective world-wide multi-centre study is now warranted.
| Item Type: | Article |
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| Additional Information: | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 19 Aug 2016 00:44 |
| Last Modified: | 10 Oct 2016 05:45 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/475 |
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