Villanueva, Jeanette E. and Gao, Ling and Dutta, Sanjay and Doyle, Aoife and Joshi, Yashutosh and Macdonald, Peter S. (2024) Using an Isolated Working Rat Heart Model to Test Donor Heart Preservation Strategies. Journal of Visualized Experiments (212). ISSN 1940-087X
Full text not available from this repository.Abstract
Optimization of donor heart preservation solutions has played a key role in reducing ischemia-reperfusion injury in donor hearts during organ retrieval, transportation, and transplantation. Previous work from our laboratory showed that the addition of glyceryl trinitrate and erythropoietin to donor heart preservation solutions could significantly improve cardiac functional recovery after prolonged cold storage and in donation after circulatory death hearts. This supplementation protocol has been implemented in clinical use in transplant units around Australia, Belgium, and the United Kingdom. Here, we outline a protocol for testing supplementation strategies using an ex vivo isolated working rat heart (IWRH) perfusion circuit. Using this methodology, supplementation strategies can be tested in the context of prolonged cold static storage, donation after brain death, and donation after circulatory death donor heart preservation. Cardiac functional recovery, measured by aortic flow, coronary flow, cardiac output, pulse pressure, and heart rate, can be used to determine whether a particular preservation strategy can minimize ischemia-reperfusion injury of the donor heart.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 02 Jan 2025 00:08 |
| Last Modified: | 02 Jan 2025 00:08 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/1619 |
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