Guarnacci, Marco and Zhang, Pei-Hong and Kanchi, Madhu and Hung, Yu-Ting and Lin, Hanrong and Shirokikh, Nikolay E and Yang, Li and Preiss, Thomas (2024) Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnover. Life Science Alliance, 7 (9). pp. e202402613. ISSN 2575-1077
Full text not available from this repository.Abstract
Maps of the RNA modification 5-methylcytosine (m(5)C) often diverge markedly not only because of differences in detection methods, data depand analysis pipelines but also biological factors. We re-analysed bisulfite RNA sequencing datasets from five human cell lines and seven tissues using a coherent m(5)C site calling pipeline. With the resulting union list of 6,393 m(5)C sites, we studied site distribution, enzymology, interaction with RNA-binding proteins and molecular function. We confirmed tRNA:m(5)C methyltransferases NSUN2 and NSUN6 as the main mRNA m(5)C "writers," but further showed that the rRNA:m(5)C methyltransferase NSUN5 can also modify mRNA. Each enzyme recognises mRNA features that strongly resemble their canonical substrates. By analysing proximity between mRNA m(5)C sites and footprints of RNA-binding proteins, we identified new candidates for functional interactions, including the RNA helicases DDX3X, involved in mRNA translation, and UPF1, an mRNA decay factor. We found that lack of NSUN2 in HeLa cells affected both steady-state levels of, and UPF1-binding to, target mRNAs. Our studies emphasise the emerging diversity of m(5)C writers and readers and their effect on mRNA function.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 23 Dec 2024 00:28 |
| Last Modified: | 23 Dec 2024 00:28 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/1556 |
Actions (login required)
 |
View Item |