Yang, Xi and Dai, Zifeng and Gao, Caixia and Yin, Yongqiang and Shi, Changbin and Liu, Renjing and Zhuge, Qichuan and Huang, Yue and Zhou, Bin and Han, Zhiming and Zheng, Xiangjian (2022) Cerebral cavernous malformation development in chronic mouse models driven by dual recombinases induced gene deletion in brain endothelial cells. Journal of Cerebral Blood Flow & Metabolism, 42 (12). pp.2230-2244. ISSN 0271-678X
Full text not available from this repository.Abstract
Cerebral cavernous malformation (CCM) is a brain vascular disease which can cause stroke, cerebral hemorrhage and neurological deficits in affected individuals. Loss-of-function mutations in three genes (CCM1, CCM2 and CCM3) cause CCM disease. Multiple mouse models for CCM disease have been developed although each of them are associated with various limitations. Here, we employed the Dre-Cre dual recombinase system to specifically delete Ccm genes in brain endothelial cells. In this new series of CCM mouse models, robust CCM lesions now develop in the cerebrum. The survival curve and lesion burden analysis revealed that Ccm2 deletion causes modest CCM lesions with a median life expectance of approximately 10 months and Ccm3 gene deletion leads to the most severe CCM lesions with median life expectance of approximately 2 months. The extended lifespan of these mutant mice enables their utility in behavioral analyses of neurologic deficits in adult mice, and allow the development of methods to quantify lesion burden in mice over time and also permit longitudinal drug testing in live animals.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 03 Mar 2023 01:17 |
| Last Modified: | 03 Mar 2023 01:17 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/1325 |
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