Roch, Aline and Magon, Nicholas J. and Maire, Jessica and Suarna, Cacang and Ayer, Anita and Waldvogel, Sophie and Imhof, Beat A. and Koury, Mark J. and Stocker, Roland and Schapira, Marc (2019) Transition to 37°C reveals importance of NADPH in mitigating oxidative stress in stored RBCs. JCI Insight, 4 (21). ISSN 2379-3708
Full text not available from this repository.Abstract
The RBC storage lesion is a multiparametric response that occurs during storage at 4°C, but its impact on transfused patients remains unclear. In studies of the RBC storage lesion, the temperature transition from cold storage to normal body temperature that occurs during transfusion has received limited attention. We hypothesized that multiple deleterious events might occur in this period of increasing temperature. We show dramatic alterations in several properties of therapeutic blood units stored at 4°C after warming them to normal body temperature (37°C), as well as febrile temperature (40°C). In particular, the intracellular content and redox state of NADP(H) were directly affected by post-storage incubation at 37°C, as well as by pro-oxidant storage conditions. Modulation of the NADPH-producing pentose phosphate pathway, but not the prevention of hemoglobin autoxidation by conversion of oxyhemoglobin to carboxyhemoglobin, provided protection against storage-induced alterations in RBCs, demonstrating the central role of NADPH in mitigating increased susceptibility of stored RBCs to oxidative stress. We propose that assessing RBC oxidative status after restoration of body temperature constitutes a sensitive method for detecting storage-related alterations that has the potential to improve the quality of stored RBCs for transfusion.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 19 Nov 2019 23:30 |
| Last Modified: | 19 Nov 2019 23:30 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/881 |
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