Baker, Matthew A. B. and Nieves, Daniel J. and Hilzenrat, Geva and Berengut, Jonathan F. and Gaus, Katharina and Lee, Lawrence K. (2019) Stoichiometric quantification of spatially dense assemblies with qPAINT. Nanoscale. ISSN 2040-3364
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Link to published document: http://doi.org/10.1039/c9nr00472f
Abstract
Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 04 Jun 2019 00:32 |
| Last Modified: | 04 Jun 2019 00:32 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/843 |
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