Farbehi, Nona and Patrick, Ralph and Dorison, Aude and Xaymardan, Munira and Janbandhu, Vaibhao and Wystub-Lis, Katharina and Ho, Joshua W K and Nordon, Robert E and Harvey, Richard P (2019) Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. eLife, 8 (epub). ISSN 2050-084X
Farbehi, Nona and Patrick, Ralph and Dorison, Aude and Xaymardan, Munira and Janbandhu, Vaibhao and Wystub-Lis, Katharina and Ho, Joshua W K and Nordon, Robert E and Harvey, Richard P (2019) Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. eLife, 8 (epub). ISSN 2050-084X
Farbehi, Nona and Patrick, Ralph and Dorison, Aude and Xaymardan, Munira and Janbandhu, Vaibhao and Wystub-Lis, Katharina and Ho, Joshua W K and Nordon, Robert E and Harvey, Richard P (2019) Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. eLife, 8 (epub). ISSN 2050-084X
Abstract
Besides cardiomyocytes (CM), the heart contains numerous stromal cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction surgery. Clustering of >30,000 single cells allowed us to identify >30 populations representing 9 cell lineages, included a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also defined three novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures, and 8 macrophage subsets. These comprehensive cardiac single cell transcriptome data provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.
Metadata
Subjects: | R Medicine > R Medicine (General) |
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Depositing User: | Repository Administrator |
Date Deposited: | 08 Apr 2019 04:03 |
Last Modified: | 08 Apr 2019 04:03 |