Murphy, Patricia and Kabir, Md Humayun and Srivastava, Tarini and Mason, Michele E. and Dewi, Chitra U. and Lim, Seakcheng and Yang, Andrian and Djordjevic, Djordje and Killingsworth, Murray C and Ho, Joshua W K and Harman, David G and O'Connor, Michael D (2018) Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro. Development, 145 (1). pp.1-12. ISSN 0950-1991 (OA)
Murphy, Patricia and Kabir, Md Humayun and Srivastava, Tarini and Mason, Michele E. and Dewi, Chitra U. and Lim, Seakcheng and Yang, Andrian and Djordjevic, Djordje and Killingsworth, Murray C and Ho, Joshua W K and Harman, David G and O'Connor, Michael D (2018) Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro. Development, 145 (1). pp.1-12. ISSN 0950-1991 (OA)
Murphy, Patricia and Kabir, Md Humayun and Srivastava, Tarini and Mason, Michele E. and Dewi, Chitra U. and Lim, Seakcheng and Yang, Andrian and Djordjevic, Djordje and Killingsworth, Murray C and Ho, Joshua W K and Harman, David G and O'Connor, Michael D (2018) Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro. Development, 145 (1). pp.1-12. ISSN 0950-1991 (OA)
Abstract
Cataracts cause vision loss and blindness by impairing the ability of the ocular lens to focus light onto the retina. Various cataract risk factors have been identified, including drug treatments, age, smoking and diabetes. However, the molecular events responsible for these different forms of cataract are ill-defined, and the advent of modern cataract surgery in the 1960s virtually eliminated access to human lenses for research. Here, we demonstrate large-scale production of light-focusing human micro-lenses from spheroidal masses of human lens epithelial cells purified from differentiating pluripotent stem cells. The purified lens cells and micro-lenses display similar morphology, cellular arrangement, mRNA expression and protein expression to human lens cells and lenses. Exposing the micro-lenses to the emergent cystic fibrosis drug Vx-770 reduces micro-lens transparency and focusing ability. These human micro-lenses provide a powerful and large-scale platform for defining molecular disease mechanisms caused by cataract risk factors, for anti-cataract drug screening and for clinically relevant toxicity assays.
Metadata
Additional Information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
---|---|
Subjects: | R Medicine > R Medicine (General) |
Depositing User: | Repository Administrator |
Date Deposited: | 09 Feb 2018 09:21 |
Last Modified: | 15 Mar 2018 05:04 |
Download
Filename: Murphy 2018 Stem cells and cataracts _Development OA.pdf