Waters, Shafagh A and Livernois, Alexandra M and Patel, Hardip and O’Meally, Denis and Craig, Jeff M and Graves, Jennifer A Marshall and Suter, Catherine M and Waters, Paul D (2018) Landscape of DNA methylation on the marsupial X. Molecular Biology and Evolution, 35 (2). pp.431-439. ISSN 0737-4038 (N/A)
Full text not available from this repository.Abstract
DNA methylation plays a key role in maintaining transcriptional silence on the inactive X chromosome of eutherian mammals. Beyond eutherians, there are limited genome wide data on DNA methylation from other vertebrates. Previous studies of X borne genes in various marsupial models revealed no differential DNA methylation of promoters between the sexes, leading to the conclusion that CpG methylation plays no role in marsupial X-inactivation. Using reduced representation bisulfite sequencing, we generated male and female CpG methylation profiles in four representative vertebrates (mouse, gray short-tailed opossum, platypus and chicken). A variety of DNA methylation patterns were observed. Platypus and chicken displayed no large-scale differential DNA methylation between the sexes on the autosomes or the sex chromosomes. As expected, a metagene analysis revealed hypermethylation at transcription start sites of genes subject to X-inactivation in female mice. This contrasted with the opossum, in which metagene analysis did not detect differential DNA methylation between the sexes at transcription start sites of genes subject to X-inactivation. However, regions flanking transcription start sites of these genes were hypomethylated. Our data are the first to demonstrate that, for genes subject to X-inactivation in both eutherian and marsupial mammals, there is a consistent difference between DNA methylation levels at transcription start sites and immediate flanking regions, which we propose has a silencing effect in both groups.
| Item Type: | Article |
|---|---|
| Additional Information: | Post print on file. |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 27 Nov 2017 01:54 |
| Last Modified: | 15 Mar 2018 21:55 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/667 |
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