Nobis, Max and Herrmann, David and Warren, Sean C. and Kadir, Shereen and Leung, Wilfred and Killen, Monica and Magenau, Astrid and Stevenson, David and Lucas, Morghan C. and Reischmann, Nadine and Vennin, Claire and Conway, James R.W. and Boulghourjian, Alice and Zaratzian, Anaiis and Law, Andrew M. and Gallego-Ortega, David and Ormandy, Christopher J. and Walters, Stacey N. and Grey, Shane T. and Bailey, Jacqueline and Chtanova, Tatyana and Quinn, Julian M.W. and Baldock, Paul A. and Croucher, Peter I. and Schwarz, Juliane P. and Mrowinska, Agata and Zhang, Lei and Herzog, Herbert and Masedunskas, Andrius and Hardeman, Edna C. and Gunning, Peter W. and Del Monte-Nieto, Gonzalo and Harvey, Richard P and Samuel, Michael S. and Pajic, Marina and McGhee, Ewan J. and Johnsson, Anna-Karin E. and Sansom, Owen J. and Welch, Heidi C.E. and Morton, Jennifer P. and Strathdee, Douglas and Anderson, Kurt I. and Timpson, Paul (2017) A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts. Cell Reports, 21 (1). pp.274-288. ISSN 22111247 (Gold OA)
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Abstract
The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance energy transfer (FRET) biosensor mouse and its utility for monitoring real-time activity of RhoA in a variety of native tissues in vivo. We assess changes in RhoA activity during mechanosensing of osteocytes within the bone and during neutrophil migration. We also demonstrate spatiotemporal order of RhoA activity within crypt cells of the small intestine and during different stages of mammary gestation. Subsequently, we reveal co-option of RhoA activity in both invasive breast and pancreatic cancers, and we assess drug targeting in these disease settings, illustrating the potential for utilizing this mouse to study RhoA activity in vivo in real time.
| Item Type: | Article |
|---|---|
| Additional Information: | This article is available under a Creative Commons license https://creativecommons.org/licenses/by/4.0/ |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 08 Oct 2017 21:38 |
| Last Modified: | 27 May 2018 22:39 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/652 |
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