Fonoudi, Hananeh and Ansari, Hassan and Abbasalizadeh, Saeed and Blue, Gillian M and Aghdami, Nasser and Winlaw, David S and Harvey, Richard P and Bosman, Alexis and Baharvand, Hossein (2016) Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol. Jove-Journal of Visualized Experiments (113). pp.1-10. ISSN 1940-087X (Not OA)
Full text not available from this repository.Abstract
Maximizing the benefit of human pluripotent stem cells (hPSCs) for research, disease modeling, pharmaceutical and clinical applications requires robust methods for the large-scale production of functional cell types, including cardiomyocytes. Here we demonstrate that the temporal manipulation of WNT, TGF-β, and SHH signaling pathways leads to highly efficient cardiomyocyte differentiation of single-cell passaged hPSC lines in both static suspension and stirred suspension bioreactor systems. Employing this strategy resulted in ~ 100% beating spheroids, consistently containing > 80% cardiac troponin T-positive cells after 15 days of culture, validated in multiple hPSC lines. We also report on a variation of this protocol for use with cell lines not currently adapted to single-cell passaging, the success of which has been verified in 42 hPSC lines. Cardiomyocytes generated using these protocols express lineage-specific markers and show expected electrophysiological functionalities. Our protocol presents a simple, efficient and robust platform for the large-scale production of human cardiomyocytes.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 19 Aug 2016 00:59 |
| Last Modified: | 16 Mar 2018 04:13 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/476 |
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