Liao, Yalin and Castello, Alfredo and Fischer, Bernd and Leicht, Stefan and Föehr, Sophia and Frese, Christian K and Ragan, Chikako and Kurscheid, Sebastian and Pagler, Eloisa and Yang, Hao and Krijgsveld, Jeroen and Hentze, Matthias W and Preiss, Thomas (2016) The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease. Cell Reports, 16 (5). pp.1456-69. ISSN 2211-1247 (OA)
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Abstract
RNA functions through the dynamic formation of complexes with RNA-binding proteins (RBPs) in all clades of life. We determined the RBP repertoire of beating cardiomyocytic HL-1 cells by jointly employing two in vivo proteomic methods, mRNA interactome capture and RBDmap. Together, these yielded 1,148 RBPs, 391 of which are shared with all other available mammalian RBP repertoires, while 393 are thus far unique to cardiomyocytes. RBDmap further identified 568 regions of RNA contact within 368 RBPs. The cardiomyocyte mRNA interactome composition reflects their unique biology. Proteins with roles in cardiovascular physiology or disease, mitochondrial function, and intermediary metabolism are all highly represented. Notably, we identified 73 metabolic enzymes as RBPs. RNA-enzyme contacts frequently involve Rossmann fold domains with examples in evidence of both, mutual exclusivity of, or compatibility between RNA binding and enzymatic function. Our findings raise the prospect of previously hidden RNA-mediated regulatory interactions among cardiomyocyte gene expression, physiology, and metabolism.
| Item Type: | Article |
|---|---|
| Additional Information: | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 19 Aug 2016 00:36 |
| Last Modified: | 19 Aug 2016 00:36 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/474 |
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