Wang, Yong and Liu, Yanxin and Deberg, Hannah A and Nomura, Takeshi and Hoffman, Melinda Tonks and Rohde, Paul R and Schulten, Klaus and Martinac, Boris and Selvin, Paul R (2014) Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel. eLife, 3. pp. e01834. ISSN 2050-084X (OA)
Full text not available from this repository.Abstract
The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements and electrophysiological sieving experiments show that the open-diameter of the channel pore is >25 Å, but the exact size and whether the conformational change follows a helix-tilt or barrel-stave model are unclear. Here we report measurements of the distance changes on liposome-reconstituted MscL transmembrane α-helices, using a 'virtual sorting' single-molecule fluorescence energy transfer. We observed directly that the channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter. In addition, based on the measurements, we developed a molecular dynamics model of the channel structure in the open state which confirms our direct observations. DOI: http://dx.doi.org/10.7554/eLife.01834.001.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 08 Feb 2016 02:47 |
| Last Modified: | 06 Jul 2016 05:31 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/375 |
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