Nakayama, Yoshitaka and Becker, Michael and Ebrahimian, Haleh and Konishi, Tomoyuki and Kawasaki, Hisashi and Krämer, Reinhard and Martinac, Boris (2016) The impact of the C-terminal domain on the gating properties of MscCG from Corynebacterium glutamicum. Biochimica et Biophysica Acta, 1858 (1). pp.130-8. ISSN 0006-3002 (PP OA)
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Abstract
The mechanosensitive (MS) channel MscCG from the soil bacterium Corynebacterium glutamicum functions as a major glutamate exporter. MscCG belongs to a subfamily of the bacterial MscS-like channels, which play an important role in osmoregulation. To understand the structural and functional features of MscCG, we investigated the role of the carboxyl-terminal domain, whose relevance for the channel gating has been unknown. The chimeric channel MscS-(C-MscCG), which is a fusion protein between the carboxyl terminal domain of MscCG and the MscS channel, was examined by the patch clamp technique. We found that the chimeric channel exhibited MS channel activity in Escherichia coli spheroplasts characterized by a lower activation threshold and slow closing compared to MscS. The chimeric channel MscS-(C-MscCG) was successfully reconstituted into azolectin liposomes and exhibited gating hysteresis in a voltage-dependent manner, especially at high pipette voltages. Moreover, the channel remained open after releasing pipette pressure at membrane potentials physiologically relevant for C. glutamicum. This contribution to the gating hysteresis of the C-terminal domain of MscCG confers to the channel gating properties highly suitable for release of intracellular solutes.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 05 Feb 2016 03:59 |
| Last Modified: | 06 Nov 2016 05:44 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/347 |
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