Yoo, Jung-Yoon and Kim, Tae Hoon and Lee, Jae Hee and Dunwoodie, Sally L and Ku, Bon Jeong and Jeong, Jae-Wook (2015) Mig-6 regulates endometrial genes involved in cell cycle and progesterone signaling. Biochemical and Biophysical Research Communications, 462 (4). pp.409-14. ISSN 1090-2104 (PMC OA)
Full text not available from this repository.Abstract
Mitogen inducible gene 6 (Mig-6) is an important mediator of progesterone (P4) signaling to inhibit estrogen (E2) signaling in the uterus. Ablation of Mig-6 in the murine uterus leads to the development of endometrial hyperplasia and E2-induced endometrial cancer. To identify the molecular pathways regulated by Mig-6, we performed microarray analysis on the uterus of ovariectomized Mig-6(f/f) and PGR(cre/+)Mig-6(f/f) (Mig-6(d/d)) mice treated with vehicle or P4 for 6 h. The results revealed that 772 transcripts were significantly regulated in the Mig-6(d/d) uterus treated with vehicle as compared with Mig-6(f/f) mice. The pathway analysis showed that Mig-6 suppressed the expression of gene-related cell cycle regulation in the absence of ovarian steroid hormone. The epithelium of Mig-6(d/d) mice showed a significant increase in the number of proliferative cells compared to Mig-6(f/f) mice. This microarray analysis also revealed that 324 genes are regulated by P4 as well as Mig-6. Cited2, the developmentally important transcription factor, was identified as being regulated by the P4-Mig-6 axis. To determine the role of Cited2 in the uterus, we used the mice with Cited2 that were conditionally ablated in progesterone receptor-positive cells (PGR(cre/+)Cited2(f/f); Cited2(d/d)). Ablation of Cited2 in the uterus resulted in a significant reduction in the ability of the uterus to undergo a hormonally induced decidual reaction. Identification and analysis of these responsive genes will help define the role of P4 as well as Mig-6 in regulating uterine biology.
| Item Type: | Article |
|---|---|
| Additional Information: | This article is available for free from the PMC website: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461536/ |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 03 Feb 2016 23:06 |
| Last Modified: | 17 Jan 2018 08:38 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/300 |
Actions (login required)
 |
View Item |