Keam, Simon P and Sobala, Andrew and Humphreys, David T and Suter, Catherine M and Hutvagner, Gyorgy (2014) Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments. Methods in Molecular Biology, 1173. pp.157-67. ISSN 1940-6029 (Book Chapter)
Full text not available from this repository.Abstract
The rapidly growing list of small RNA species generated by next-generation sequencing technologies has accelerated the development of new bioinformatics tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation sequencing libraries. We also present a biochemical purification protocol for enriching 5' tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 27 Jan 2016 03:28 |
| Last Modified: | 27 May 2016 06:51 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/251 |
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