Acera Mateos, P and J Sethi, A and Ravindran, A and Srivastava, A and Woodward, K and Mahmud, S and Kanchi, M and Guarnacci, M and Xu, J and W S Yuen, Z and Zhou, Y and Sneddon, A and Hamilton, W and Gao, J and M Starrs, L and Hayashi, R and Wickramasinghe, V and Zarnack, K and Preiss, T and Burgio, G and Dehorter, N and E Shirokikh, N and Eyras, E (2024) Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications. Nature Communications, 15 (1). ISSN 2041-1723
Full text not available from this repository.Abstract
The epitranscriptome embodies many new and largely unexplored functions of RNA. A significant roadblock hindering progress in epitranscriptomics is the identification of more than one modification in individual transcript molecules. We address this with CHEUI (CH3 (methylation) Estimation Using Ionic current). CHEUI predicts N6-methyladenosine (m6A) and 5-methylcytosine (m5C) in individual molecules from the same sample, the stoichiometry at transcript reference sites, and differential methylation between any two conditions. CHEUI processes observed and expected nanopore direct RNA sequencing signals to achieve high single-molecule, transcript-site, and stoichiometry accuracies in multiple tests using synthetic RNA standards and cell line data. CHEUI's capability to identify two modification types in the same sample reveals a co-occurrence of m6A and m5C in individual mRNAs in cell line and tissue transcriptomes. CHEUI provides new avenues to discover and study the function of the epitranscriptome.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 17 Dec 2024 22:08 |
| Last Modified: | 17 Dec 2024 22:08 |
| URI: | https://vceprints02.victorchang.edu.au/id/eprint/1543 |
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