Yang, Xi and Wu, Shi-Ting and Gao, Rui and Wang, Rui and Wang, Yixuan and Dong, Zhenkun and Wang, Lu and Qi, Chunxiao and Wang, Xiaohong and Schmitz, M. Lienhard and Liu, Renjing and Han, Zhiming and Wang, Lu and Zheng, Xiangjian (2023) Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers cerebral cavernous malformation. JCI Insight, 8 (5). ISSN 2379-3708
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Release-of-STK24-25-suppression-on-MEKK3-signaling-in-endothelial-cells-confers-cerebral-cavernous-malformation.pdf Available under License Creative Commons Attribution No Derivatives. Download (26MB) | Preview |
Abstract
Loss-of-function mutations in cerebral cavernous malformation (CCM) genes and gain-of-function mutation in the MAP3K3 gene encoding MEKK3 cause CCM. Deficiency of CCM proteins leads to the activation of MEKK3-KLF2/4 signaling, but it is not clear how this occurs. Here, we demonstrate that deletion of the CCM3 interacting kinases STK24/25 in endothelial cells causes defects in vascular patterning during development as well as CCM lesion formation during postnatal life. While permanent deletion of STK24/25 in endothelial cells caused developmental defects of the vascular system, inducible postnatal deletion of STK24/25 impaired angiogenesis in the retina and brain. More importantly, deletion of STK24/25 in neonatal mice led to the development of severe CCM lesions. At the molecular level, a hybrid protein consisting of the STK kinase domain and the MEKK3 interacting domain of CCM2 rescued the vascular phenotype caused by the loss of ccm gene function in zebrafish. Our study suggests that CCM2/3 proteins act as adapters to allow recruitment of STK24/25 to limit the constitutive MEKK3 activity, thus contributing to vessel stability. Loss of STK24/25 causes MEKK3 activation, leading to CCM lesion formation.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 10 Jul 2023 04:29 |
| Last Modified: | 10 Jul 2023 05:39 |
| URI: | http://eprints.victorchang.edu.au/id/eprint/1426 |
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