Nakano, Haruko and Liu, Xiaoqian and Arshi, Armin and Nakashima, Yasuhiro and van Handel, Ben and Sasidharan, Rajkumar and Harmon, Andrew W and Shin, Jae-Ho and Schwartz, Robert J and Conway, Simon J and Harvey, Richard P and Pashmforoush, Mohammad and Mikkola, Hanna K A and Nakano, Atsushi (2013) Haemogenic endocardium contributes to transient definitive haematopoiesis. Nature Communications, 4. p. 1564. ISSN 2041-1723 (OA)
Preview |
Text
Nakano 2013 Haemogenic endocard _Nature Comms OA.pdf Download (2MB) | Preview |
Abstract
Haematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial and haematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a haemogenic organ akin to the dorsal aorta. Here we examine the haemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Haemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2-5 and Isl1, are expressed in and required for the haemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells serve as a de novo source for transient definitive haematopoietic progenitors.
(Grants from BSCRC, AHA (IRG4870007, GRNT9420039) and NIH (R21HL109938, R01HL097766); HHMI undergraduate fellowship)
| Item Type: | Article |
|---|---|
| Additional Information: | DOAJ publication |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 07 Jan 2016 03:07 |
| Last Modified: | 18 May 2016 23:47 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/132 |
Actions (login required)
 |
View Item |