Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing

Sibbritt, Tennille and Schumann, Ulrike and Shafik, Andrew and Guarnacci, Marco and Clark, Susan J. and Preiss, Thomas (2022) Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing. Methods Mol Biol, 2404. pp.375-392. ISSN 1064-3745

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Link to published document: http://doi.org/10.1007/978-1-0716-1851-6_21

Abstract

Mapping the position and quantifying the level of 5-methylcytosine (m(5)C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m(5)C in DNA, but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification, and detection of candidate sites by sequencing on the Illumina MiSeq platform.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Repository Administrator
Date Deposited: 14 Apr 2022 03:56
Last Modified: 19 Apr 2022 00:40
URI: http://eprints.victorchang.edu.au/id/eprint/1218

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