Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration

Luo, Kaiming and Ogawa, Masahito and Ayer, Anita and Britton, Warwick J. and Stocker, Roland and Kikuchi, Kazu and Oehlers, Stefan H. (2022) Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration. Zebrafish, 19 (1). pp.7-17. ISSN 1545-8547

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Link to published document: http://doi.org/10.1089/zeb.2021.0058

Abstract

Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish hmox1a and characterize the effects of hmox1a and hmox1b loss on embryonic development. We find that loss of hmox1a or hmox1b causes developmental defects in only a minority of embryos, in contrast to Hmox1 gene deletions in mice that cause loss of most embryos. Using a tail wound inflammation assay we find a conserved role for hmox1a, but not hmox1b, in normal macrophage migration to the wound site. Together our results indicate that zebrafish hmox1a has clearly a partitioned role from hmox1b that is more consistent with conserved functions of mammalian Heme oxygenase 1.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Repository Administrator
Date Deposited: 14 Apr 2022 03:25
Last Modified: 14 Apr 2022 03:25
URI: http://eprints.victorchang.edu.au/id/eprint/1204

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