A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals

Schaniel, Christoph and Dhanan, Priyanka and Hu, Bin and Xiong, Yuguang and Raghunandan, Teeya and Gonzalez, David M. and Dariolli, Rafael and D'Souza, Sunita L. and Yadaw, Arjun S. and Hansen, Jens and Jayaraman, Gomathi and Mathew, Bino and Machado, Moara and Berger, Seth I. and Tripodi, Joseph and Najfeld, Vesna and Garg, Jalaj and Miller, Marc and Surlyn, Colleen S. and Michelis, Katherine C. and Tangirala, Neelima C. and Weerahandi, Himali and Thomas, David C. and Beaumont, Kristin G. and Sebra, Robert and Mahajan, Milind and Schadt, Eric and Vidovic, Dusica and Schürer, Stephan C. and Goldfarb, Joseph and Azeloglu, Evren U. and Birtwistle, Marc R. and Sobie, Eric A. and Kovacic, Jason C. and Dubois, Nicole C. and Iyengar, Ravi (2021) A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals. Stem Cell Reports, 16 (12). pp.3036-3049. ISSN 22136711

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Link to published document: http://doi.org/10.1016/j.stemcr.2021.10.005

Abstract

A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotype association studies, and drug response evaluation. We report generation and extensive characterization of a gender-balanced, racially/ethnically diverse library of hiPSC lines from 40 clinically healthy human individuals who range in age from 22 to 61 years. The hiPSCs match the karyotype and short tandem repeat identities of their parental fibroblasts, and have a transcription profile characteristic of pluripotent stem cells. We provide whole-genome sequencing data for one hiPSC clone from each individual, genomic ancestry determination, and analysis of mendelian disease genes and risks. We document similar transcriptomic profiles, single-cell RNA-sequencing-derived cell clusters, and physiology of cardiomyocytes differentiated from multiple independent hiPSC lines. This extensive characterization makes this hiPSC library a valuable resource for many studies on human biology.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Repository Administrator
Date Deposited: 08 Apr 2022 04:30
Last Modified: 17 Jun 2022 03:38
URI: http://eprints.victorchang.edu.au/id/eprint/1192

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