FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles

Farbehi, Nona and Janbandhu, Vaibhao and Nordon, Robert and Harvey, Richard (2021) FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles. BIO-PROTOCOL, 11 (10). pp. e4028. ISSN 2331-8325

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Link to published document: http://doi.org/10.21769/BioProtoc.4028

Abstract

Besides cardiomyocytes, the heart contains numerous interstitial cell types, including cardiac fibroblasts, endothelial cells, immune (myeloid and lymphoid) cells, and mural cells (pericytes and vascular smooth muscle cells), which play key roles in heart repair, regeneration, and disease. We recently published a comprehensive map of cardiac stromal cell heterogeneity and flux in healthy and infarcted hearts using single-cell RNA sequencing (scRNA-seq) ( Farbehi et al., 2019 ). Here, we describe the FACS (Fluorescent Activated Cell Sorting)-based method used in that study for isolation of two cardiac cell fractions from adult mouse ventricles: the total interstitial cell population (TIP; non-cardiomyocytes) and enriched (Pdgfra-GFP(+)) cardiac fibroblasts.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Repository Administrator
Date Deposited: 31 Jul 2021 04:22
Last Modified: 31 Jul 2021 04:22
URI: http://eprints.victorchang.edu.au/id/eprint/1079

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