Romanov, Valentin and Silvani, Giulia and Zhu, Huiyu and Cox, Charles D. and Martinac, Boris (2021) An Acoustic Platform for Single‐Cell, High‐Throughput Measurements of the Viscoelastic Properties of Cells. Small, 17 (3). p. 2005759. ISSN 1613-6810
Full text not available from this repository.Abstract
Cellular processes including adhesion, migration, and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. Here, single-cell, high-throughput characterization of adherent cells is demonstrated using acoustic force spectroscopy (AFS). AFS works by simultaneously, acoustically driving tens to hundreds of silica beads attached to cells away from the cell surface, allowing the user to measure the stiffness of adherent cells under multiple experimental conditions. It is shown that cells undergo marked changes in viscoelasticity as a function of temperature, by altering the temperature within the AFS microfluidic circuit between 21 and 37 degrees C. In addition, quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface are shown. Further, the high-throughput format of the AFS is utilized to rapidly probe, in excess of 1000 cells, three different cell lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 15 Mar 2021 04:57 |
| Last Modified: | 15 Mar 2021 04:57 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/1049 |
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