Kuchel, Philip W. and Romanenko, Konstantin and Shishmarev, Dmitry and Galvosas, Petrik and Cox, Charles D. (2021) Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy. Scientific Reports, 11 (1). ISSN 2045-2322
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Kuchel et al - Scientific Reports - 2021.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (2MB) | Preview |
Abstract
We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca(2+) ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca(2+) chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5FBAPTA), and recorded (19)F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and (13)C NMR spectroscopy with [1,6-(13)C]D-glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca(2+) influx is concluded to be via the mechanosensitive cation channel Piezo1. The increased rate of influx brought about by the activator of Piezo1, 2-[5-[[(2,6-dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]-pyrazine (Yoda1) supported this conclusion; while the specificity of the cation-sensing by 5FBAPTA was confirmed by using the Ca(2+) ionophore, A23187.
| Item Type: | Article |
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| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 26 Feb 2021 02:54 |
| Last Modified: | 01 Aug 2021 04:19 |
| URI: | http://eprints.victorchang.edu.au/id/eprint/1039 |
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