Martin, Ella M.M.A. and Drover, Kyle and Shpak, Anton and Horner, Neil R. and O'Reilly, Victoria and Cocking, Emma and Greasby, Joelene A. and Iyer, Kavitha R. and Dunwoodie, Sally L. and Winlaw, David S. and Giannoulatou, Eleni and Nassar, Natasha and Kirk, Edwin and Arkell, Ruth and Dunwoodie, Sally L. and Chapman, Gavin (2026) Comprehensive defect detection in mouse embryos and the heart by combining automated phenotyping with novel population averages and atlases. Differentiation, 148. p. 100936. ISSN 03014681
Full text not available from this repository.Abstract
Micro-CT has become the standard for the assessment of malformations in mouse embryos because it allows the visualisation of internal structures in the context of the intact embryo. Statistical comparison of volume differences is possible via manual segmentation of organs of interest from micro-CT scans, but this process is slow and laborious. Automated registration-based methods now exist that make the volumetric analysis of all organs feasible. Here, we expand the available atlases for use with the LAMA registration and analysis pipeline to include high-resolution population averages derived from phosphotungstic acid-stained C57BL/6J embryos and corresponding manually segmented atlases at embryonic stage (E) 12.5, E15.5, and E17.5. We report application of these population averages and atlases with the LAMA phenotyping pipeline to Wbp11 heterozygous null embryos, identifying defects previously reported in the cervical vertebrae, brain, nasal cavity, palate, liver and kidneys as well as a right aortic arch defects missed by manual analysis, and volume differences in the eyes and spinal cord. Finally, we report a high-resolution isolated E18.5 mouse heart population average and corresponding atlas that when applied to the Wbp11 line identified significant differences. These findings highlight the advantages of unbiased, volumetric and quantitative approaches in the analysis of mouse models of human disease.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 29 Aug 2026 04:07 |
| Last Modified: | 29 Aug 2026 04:07 |
| URI: | http://eprints.victorchang.edu.au/id/eprint/1807 |
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