King-Hudson, Te-Rina J. and Davies, James S. and Quan, Senwei and Currie, Michael J. and Tillett, Zachary D. and Copping, Jack and Panjikar, Santosh and Friemann, Rosmarie and Allison, Jane R. and North, Rachel A. and Dobson, Renwick C.J. (2024) On the function of TRAP substrate-binding proteins: Conformational variation of the sialic acid binding protein SiaP. Journal of Biological Chemistry, 300 (11). p. 107851. ISSN 00219258
Full text not available from this repository.Abstract
Tripartite ATP-independent periplasmic (TRAP) transporters are analogous to ABC transporters in that they use a substrate-binding protein to scavenge metabolites (e.g., N-acetylneuraminate) and deliver them to the membrane components for import. TRAP substrate-binding proteins are thought to bind the substrate using a two-state (open and closed) induced-fit mechanism. We solved the structure of the TRAP N-acetylneuraminate substrate-binding protein from Aggregatibacter actinomycetemcomitans (AaSiaP) in both the open ligand-free and closed liganded conformations. Surprisingly, we also observed an intermediate conformation, where AaSiaP is mostly closed and is bound to a non-cognate ligand, acetate, which hints at how N-acetylneuraminate binding stabilizes a fully closed state. AaSiaP preferentially binds N-acetylneuraminate (K(D) = 0.4 muM) compared to N-glycolylneuraminate (K(D) = 4.4 muM), which is explained by the closed-N-acetylneuraminate bound structure. Small-angle X-ray scattering data alongside molecular dynamics simulations suggest the AaSiaP adopts a more open state in solution than in a crystal. However, the open unliganded conformation can also sample closed conformations. Molecular dynamics simulations also demonstrate the importance of water molecules for stabilizing the closed conformation. Although our data is consistent with an induced fit model of binding, we suggest that the open unliganded conformation may sample multiple states capable of binding substrate. The mechanism by which the ligand is released for import remains to be determined.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Repository Administrator |
| Date Deposited: | 02 May 2025 04:47 |
| Last Modified: | 02 May 2025 04:47 |
| URI: | https://eprints.victorchang.edu.au/id/eprint/1634 |
Actions (login required)
 |
View Item |